Journal: Cell reports

Article Title: Jedi-1/MEGF12-mediated phagocytosis controls the pro-neurogenic properties of microglia in the ventricular-subventricular zone

doi: 10.1016/j.celrep.2023.113423

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: A beveled glass needle was pre-loaded with mineral oil and the recombinant human IL-1 receptor inhibitor (IL-1RA, R&D Systems, Cat #280-RA, resuspended in 0.1% BSA), then positioned in the injecting rig at a 90° angle relative to the horizontal plane of the Petri dish and head mold.

Techniques: Recombinant, Marker, In Situ, RNA Sequencing, Knock-Out, Mutagenesis, Real-time Polymerase Chain Reaction, Software

Journal: The Journal of Experimental Medicine

Article Title: Influence of Polymorphism in the Genes for the Interleukin (IL)-1 Receptor Antagonist and IL-1β on Tuberculosis

doi:

Figure Lengend Snippet: Association between IL-1Ra genotype and the monocyte production of IL-1Ra in response to M . tuberculosis and PPD. The amount of IL-1Ra produced by PBMCs from healthy, PPD-nonreactive donors during a 10-h coculture with either M . tuberculosis (A) or PPD (B) was determined by ELISA. Values were normalized to the number of monocytes present in the culture. The response of IL-1Ra A2 + individuals was higher at all doses of either stimuli.

Article Snippet: After washing (×4), 100 μl of the following biotinylated detection antibodies were added in diluent (0.1% BSA, 0.05% Tween 20 in TBS, pH 7.3): 100 ng/ml anti–human IL-1β antibody or 20 ng/ml anti–human IL-1Ra antibody (both from R & D Systems, Inc.).

Techniques: Produced, Enzyme-linked Immunosorbent Assay

Journal: The Journal of Experimental Medicine

Article Title: Influence of Polymorphism in the Genes for the Interleukin (IL)-1 Receptor Antagonist and IL-1β on Tuberculosis

doi:

Figure Lengend Snippet: Relationship between polymorphism in IL-1Ra and the IL-1Ra/IL-1β ratio. The IL-1β content of the supernates shown in Fig. was also assayed by ELISA. The molar ratio of IL-1Ra/IL-1β was calculated. This ratio is selectively increased in IL-1Ra A2 + individuals in response to PPD and M . tuberculosis , accentuated by the finding that IL-1Ra A2 + subjects tended to produce less IL-1β in response to M . tuberculosis . By comparison, the ratio in response to LPS was not different between IL-1Ra A2 + and A2 − subjects. Response to PPD is data from eight individuals.

Article Snippet: After washing (×4), 100 μl of the following biotinylated detection antibodies were added in diluent (0.1% BSA, 0.05% Tween 20 in TBS, pH 7.3): 100 ng/ml anti–human IL-1β antibody or 20 ng/ml anti–human IL-1Ra antibody (both from R & D Systems, Inc.).

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

Journal: The Journal of Experimental Medicine

Article Title: Influence of Polymorphism in the Genes for the Interleukin (IL)-1 Receptor Antagonist and IL-1β on Tuberculosis

doi:

Figure Lengend Snippet: Mean Fold Induction of the IL-1Rα and IL-1β Genes in Response to M. tuberculosis

Article Snippet: After washing (×4), 100 μl of the following biotinylated detection antibodies were added in diluent (0.1% BSA, 0.05% Tween 20 in TBS, pH 7.3): 100 ng/ml anti–human IL-1β antibody or 20 ng/ml anti–human IL-1Ra antibody (both from R & D Systems, Inc.).

Techniques:

Journal: The Journal of Experimental Medicine

Article Title: Influence of Polymorphism in the Genes for the Interleukin (IL)-1 Receptor Antagonist and IL-1β on Tuberculosis

doi:

Figure Lengend Snippet: (A) Effect of IL-6 and IL-10 on the IL-1Ra/IL-1β ratio. PBMCs were cultured with M . tuberculosis in the presence or absence of rhIL-6 or rhIL-10 over a dose range of 0.1–10 ng/ml. Culture supernates were assayed for IL-1Ra and IL-1β and the molar ratio calculated. IL-10 increased the ratio significantly even at the lowest dose ( P < 0.02), whereas rhIL-6 had no significant effect. (B) Effect of IFN-γ and IL-4 on the IL-1Ra/IL-1β ratio. PBMCs were cultured with M . tuberculosis in the presence or absence of rhIFN-γ or rhIL-4 over a dose range of 0.1–10 ng/ml. Culture supernates were assayed for IL-1Ra and IL-1β and the molar ratio calculated. IL-4 increased the ratio markedly even at the lowest dose ( P < 0.01), whereas higher doses of IFN-γ were required to cause a significant reduction.

Article Snippet: After washing (×4), 100 μl of the following biotinylated detection antibodies were added in diluent (0.1% BSA, 0.05% Tween 20 in TBS, pH 7.3): 100 ng/ml anti–human IL-1β antibody or 20 ng/ml anti–human IL-1Ra antibody (both from R & D Systems, Inc.).

Techniques: Cell Culture

Journal: The Journal of Experimental Medicine

Article Title: Influence of Polymorphism in the Genes for the Interleukin (IL)-1 Receptor Antagonist and IL-1β on Tuberculosis

doi:

Figure Lengend Snippet: Lack of Relationship between IL-1Ra Polymorphism and the Intracellular Growth of M. tuberculosis In Vitro

Article Snippet: After washing (×4), 100 μl of the following biotinylated detection antibodies were added in diluent (0.1% BSA, 0.05% Tween 20 in TBS, pH 7.3): 100 ng/ml anti–human IL-1β antibody or 20 ng/ml anti–human IL-1Ra antibody (both from R & D Systems, Inc.).

Techniques: Infection

Journal: The Journal of Experimental Medicine

Article Title: Influence of Polymorphism in the Genes for the Interleukin (IL)-1 Receptor Antagonist and IL-1β on Tuberculosis

doi:

Figure Lengend Snippet: IL-1Ra and IL-1β Allele and Genotype Frequencies in Tuberculosis Patients and Tuberculin-reactive Healthy Control Subjects

Article Snippet: After washing (×4), 100 μl of the following biotinylated detection antibodies were added in diluent (0.1% BSA, 0.05% Tween 20 in TBS, pH 7.3): 100 ng/ml anti–human IL-1β antibody or 20 ng/ml anti–human IL-1Ra antibody (both from R & D Systems, Inc.).

Techniques: Control

Journal: The Journal of Experimental Medicine

Article Title: Influence of Polymorphism in the Genes for the Interleukin (IL)-1 Receptor Antagonist and IL-1β on Tuberculosis

doi:

Figure Lengend Snippet: Relationship between IL-1Ra/IL-1β Haplotype and Disease Phenotype

Article Snippet: After washing (×4), 100 μl of the following biotinylated detection antibodies were added in diluent (0.1% BSA, 0.05% Tween 20 in TBS, pH 7.3): 100 ng/ml anti–human IL-1β antibody or 20 ng/ml anti–human IL-1Ra antibody (both from R & D Systems, Inc.).

Techniques:

Journal: The Journal of Experimental Medicine

Article Title: Influence of Polymorphism in the Genes for the Interleukin (IL)-1 Receptor Antagonist and IL-1β on Tuberculosis

doi:

Figure Lengend Snippet: Association between IL-1Ra haplotype and the cutaneous response to PPD of M . tuberculosis . The mean diameter of induration in response to one tuberculin unit of PPD in tuberculosis patients of various genotypes is shown. The proportion of IL-1Ra A2 + was highest (60%) in patients with an absent response and lowest in the category 21–30 mm (25%), falling gradually to its overall frequency (41%) as successively higher grades of Mantoux were considered.

Article Snippet: After washing (×4), 100 μl of the following biotinylated detection antibodies were added in diluent (0.1% BSA, 0.05% Tween 20 in TBS, pH 7.3): 100 ng/ml anti–human IL-1β antibody or 20 ng/ml anti–human IL-1Ra antibody (both from R & D Systems, Inc.).

Techniques:

Journal:

Article Title: Computational Design and Application of Endogenous Promoters for Transcriptionally Targeted Gene Therapy for Rheumatoid Arthritis

doi: 10.1038/mt.2009.182

Figure Lengend Snippet: Comparison of therapeutic efficacy using Saa3 versus IL1E/IL6P promoter for disease-regulated Il1rn expression. (a) Promoter activity in transduced synovium of naive and arthritic C57Bl/6 mice. Knee joints were injected with 300 ng p24gag equivalent lentivirus encoding PGK, Saa3, or IL1E/IL6P-luciferase. Seven days after transduction, arthritis was induced by intra-articular injection of 180 µg zymosan A. After 24 hours, luciferase activity was assessed ex vivo. Data are represented as individual relative luciferase activities; horizontal bars indicate the means per group. (b) Efficacy of transcriptionally targeted adenoviral vectors expressing Il1rn. NIH-3T3-5xNF-κB-Luc were transduced at a multiplicity of infection (MOI) of 10 with control vector (del) or adenovirus encoding CMV, Saa3, and IL1E/IL6P-driven Il1rn. After 24 hours, cells were transduced at an MOI of 10 with control vector (del) or Ad5.CMV-Il1b. The day thereafter, IL-1β-induced NF-κB activation was assessed by luciferase assay. Data are represented as relative luciferase activities ± SEM (n = 4), and differences were determined using analysis of variance with Dunnett's post-test. **P < 0.01.

Article Snippet: White high-binding flat bottom 96-well plates (Greiner Bio-One, Alphen a/d Rijn, the Netherlands) were coated with the capture antibody rat anti-murine Il1rn (MAB480; R&D Systems, Minneapolis, MN) at 3 μg/ml in 0.1 mol/l carbonate buffer pH 9.6 and incubated overnight at 4 °C.

Techniques: Comparison, Drug discovery, Expressing, Activity Assay, Injection, Luciferase, Transduction, Ex Vivo, Infection, Control, Plasmid Preparation, Activation Assay